Out of all the cancers colon cancer is one of the most common cancers in the world. Every year 1.2 million patients are diagnosed for colon cancer. The rate of colon cancer incidence was low in India but is presently increasing; out of 3.5 million cancer cases, 35,000 suffer from colon cancer. Phytochemically the plant has been investigated for cardenolides, alkaloids, triterpenes and saponins and it is found to contain a variety of triterpenes and steroidal compounds and also to find out, a newer synthetic drug, for its anti-colon cancer potential and its toxic profile. Two formulations were prepared using herbs and they were compared for their anti-cancer activity. Two kinds of polyherbal formulations were prepared using different extracts and they were tested for anti-cancer activity invitro. Formulations with potent drugs like ashwagandha and colchicum showed a potent activity than other formulation. Before the clinical usage of extract, thorough toxicological profile has to be determined on the crude extracts as well as on isolated compounds to confirm the safety of the drug.