ABSTRACT Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A simple, rapid, precise, and reliable reverse phase HPLC method was developed for the separation and estimation of three drugs Lamivudine, Abacavir And Zidovudine in bulk drug mix and pharmaceutical dosage forms. The estimation was carried out using Inertsil ODS 250mm x 4.6 mm, 5μ. column; mobile phase consisting of Buffer, Acetonitrile and methanol 65:15:20; the flow rate of 1 mL/min and ultraviolet detection at 250 nm. All the three drugs were properly resolved having run time of 2.1 min, 2.4 min and 6.9 min for Lamivudine, Abacavir And Zidovudine, respectively. The method was validated as a final verification of method development with respect to precision, linearity, accuracy, ruggedness, and robustness. The validated method was successfully applied to the commercially available pharmaceutical dosage form, yielding very good and reproducible result. Key words: Lamivudine, Abacavir, Zidovudine, Reverse phase HPLC.

ABSTRACT Medicinal plants are in use for long to cure ailments in the tribal area. Andrographis paniculata is selected for the antibacterial and phytochemical analysis on the basis of medicinal folklore reports and literature data. A. paniculata of Acanthaceae is an annual herb which is widely cultivated in Southern Asia, India, China and some parts of Europe. The extracts of Acetone, aqueous, chloroform, ethanol, methanol and petroleum ether solvents were subjected to qualitative phytochemical screening and antibacterial activity using standard procedures. Results showedthe presence of phytoconstituents such as Alkaloids, Glycoside, carbohydrate, flavonoids, phenols, saponins, sterols, terpenoids and tannins. Among thirteen bacterial strains tested, eight were found to be positive while the others were negative. The highest activity was recorded in methanol extract followed by ethanol, chloroform, acetone and aqueous (Table 2, Chart 1, Plate 1). The diversity of phytochemicals present suggests that A.paniculata could serve as a source of useful drugs. Key words: Antibacterial, Phytochemical, Medicinal Plant, Andrographis paniculata (Burm. fil.) Nees.

ABSTRACT In recent years there has been an increasing trend in self medication with over the counter (OTC) drugs available in pharmacies and retail outlets. In parallel, more products have been deregulated for purchase without prescription.In India, the Drugs and Cosmetics Act (DCA), the Drugs and Cosmetics Rules, 1945 (DCR) regulates the import, manufacture, distribution and sale of drugs and cosmetics. The “OTC Drugs” has no legal implications in India.To assess the awareness and attitude towards over the counter (OTC) drugs amongst urban population Chidambaram. This is cross-sectional study was carried out in urban Chidambaram. 64% of the primary respondents felt that OTC medication can be taken and 4% stated that OTC can be taken during emergencies only and 32% were of the opinion that OTC shouldn‟t be taken. 32% participants reported that they never took, 66.4% of the respondents reported they seldom take OTC medication and 1.6% of the participants reported that they always take OTC medication. Over the counter (OTC) drugs are widely used and so it is very difficult to restrict them. Education programmes for the public and the professionals will be very much necessary and compulsory. Key words: Over the counter drugs, Awareness and attitude.

ABSTRACT The objective of this work was to investigate feasibility of transdermal and dermal delivery of Dexibuprofen using porcine skin was investigated. The total flux of Dexibuprofen from the skin remarkably varied over the pH range 3.0–8.0. Transport of Dexibuprofen through and into porcine skin and effects of pH was studied ex-vivo using Franz diffusion cell. From aqueous donor samples, Dexibuprofen flux through the skin was 29.71±1.13 μg/cm2/ hr with greatest permeation rate at pH 7.0. The skin permeability data were calculated. The result of this studies showed that the total flux increased with increase of pH was dependent on the lipophilicity of drug. These results suggest that the pH of formulation should be given attention to elicit the maximum permeation enhancement through the skin. Key words: Dexibuprofen, pH, Skin permeation, Transdermal drug delivery system.

ABSTRACT Stroke is the second leading cause of death and major cause of morbidity worldwide. To find out the prevalence rate of stroke among the rural population in Tamilnadu and to describe the risk factors profile of stroke patients. A descriptive cross sectional study was carried out using predesigned structured proforma. The prevalence of stroke among the rural population Orathur in the year 2014 was found to be 1.5 per 1000. Out of 73 cases of stroke 96 % had hemiplegia. Male to female ratio of stroke cases was 8:1. 59% of them were obese. Out of the 73 cases, 69 had atleast anyone of the comorbid conditions. 74% had hypertension, 57.5% had diabetes, 11% had cardiac diseases. 4.1% had all the three co-morbid conditions. The prevalence of stroke in the rural population, present study appears to be less than in many other countries, this may be due to lower life expectancy in India with a larger younger group population than in developed countries. Key words: Prevalence, Stroke, Risk factors.

ABSTRACT The fast disintegrating oral films of Loratadine are prepared using HPMC E15cps, Eudragit and PVP individully and in combination of secondary polymers like MCC, PEG 400, in different ratios with suitable plasticizer like propylene glycol and sweetener like aspartame by solvent casting method. Among the formulations F1-F6, prepared using single polymer, the formulation F2 showed good drug release of 83.55±0.65% in 10 min and the In vitro disintegration time was found to be 59.33±4.04 sec. Among the formulations F7-F12, prepared using combination of the polymers, formulation F12 showed better drug release of 99.14±0.15 % in 6 min and the disintegration time was found to be 56.33±4.04 % sec. Formulation F12 is considered as optimum due to its good In-vitro dissolution and maximum drug release compare to other formulations. Along with F12, F9 showed good in vitro results. Key words: Disintegration test, Loratadine, HPMC E15cps, EUDRAGIT, PVP.

ABSTRACT An opinion study about pharmacovigilance was carried out to identify the reasons of under-reporting. The target population was health professionals. A randomized representative sample of 500 health professionals was selected from the database of 4000 prescribers and pharmacists approved by the public health insurance. The topics under consideration were training, experience, obstacles and suggestion to improve pharmacovigilance in Cote d’Ivoire. We obtained 31.4% of responders. Physicians represented 93.6% followed by dentists (5.7%) and pharmacists (0.7%). Most of them came from urban zones (94.2%) and worked in public hospitals (97.1%). Only 11.2% of participants had a pharmacovigilance training. Twelve (8%) had reported adverse drug reactions to pharmaceutical facilities (66.7%), to Health Minister (16.7%) and to Clinical Pharmacology Department (16.7%). According to them, the spontaneous reporting problems identified were the lack of knowledge about pharmacovigilance organization (69.1%), ignorance of interest in reporting (21.9%) or lack of training (5%). In order to improve the reporting system, health professionals suggested having report forms and phone numbers in hospital. They also suggested the involvement of the heath district (75%) in monitoring adverse drug reactions system. So pharmacovigilance should hold a salient place in health care system of Cote d’Ivoire and must be closer to health professionals to enhance reporting. Key words: Knowledge - Spontaneous reporting - Opinion - Adverse drug reaction –Pharmacovigilance.

The purpose of the present study to develop an optimized gastric floating drug delivery system (GFDDS) to prolong the gastric residence time after oral administration, at a particular site and controlling the release of drug especially useful for achieving controlled plasma level as well as improving bioavailability. Cimetidine hydrochloride is a histamine H2-receptor antagonist that inhibits stomach acid production. It is commonly used in treatment of peptic ulcer disease (PUD) and gastro esophageal reflux disease (GERD). Cimetidine is also used alongside fexofenadine and other antihistamines for the treatment of skin conditions such as hives. The proposed work is envisaged to carry out the preformulation, optimization, development of in-situ orifice forming floating tablet and evaluation of floating tablet. Floating mucho adhesive tablet of cimetidine is the one which suit the concept of better patient compliance, delayed release, more efficacies and enough bioavailability to show required pharmacological action and less gastrointestinal side effects. The sustained realese floating-muchoadhesive tablet system was successfully developed and evaluated. Key words: Preformulation, Muchoadhesive, Gastrointestin.

ABSTRACTThe present study was aimed to study the antipyretic activity of the methanol extract of Chaetomorpha litorea Harveycollected from Koothankuzhi coast, Tamil Nadu, India on albino mice. Paracetamol (10mg/kg) was used as standard drug. Theantipyretic activity of Chaetomorpha litorea Harvey was estimated by Brewer’s yeast induced pyrexia on albino mice. Thevarious methanol extract doses used were 200mg/kg and 400mg/kg body weight of mice. 400mg/kg methanol extract ofChaetomorpha litorea Harvey was found to be more significant decrease in body temperature while 200mg/kg methanol extractshowed less effect. 400mg/kg methanol extract exhibited closely significant (p<0.05) decrease in elevated body temperature ascompared to standard drug. From this study, it was known that the methanol crude extract of Chaetomorpha litorea Harvey canbe used as antipyretic agent.Key words: Green seaweed, Chaetomorpha litorea, Methanol extract, Antipyretic.