Pyrazolines are the five-membered heterocyclic having two adjacent nitrogen atoms in the ring with single endocyclic double bond and are basic in nature. Pyrazoline, among the various 5-membered heterocyclic compounds have been dragging the attention because of their pharmacological actions exhibited by these compounds. Pyrazoline derivatives display many tremendous biological actions and activities including antidiabetic, anticancer, anti-hypertensive, antiprotozoal, antitumour, anti-HIV, antidepressant and anticonvulsant activities. The study of pyrazoline derivatives towards biological evaluation been an interesting field of pharmaceutical chemistry. Diabetes is a group of metabolic disorder that are characterized by the high blood glucose level which are characterized by hyperglycemia that results from the defect in insulin secretion. Comparison of standard insulin with compounds with phenyl ring or pyrazoline has been done for evaluating the decrease in the glucose level. Cancer is not a single disease, but a combination of large group of diseases which are characterized by the uncontrolled and rapid and pathological proliferation of abnormally transferred cells. Pyrazoline have wide application in the treatment of various cancer types including mouth, oesophagus, brain and bone. Synthesized pyrazoline substituted benzene sulfonyl urea, 1,3thiazole derivatives has been then screened for their potential antiproliferative and in-vitro anticancer evaluation.